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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using CoraLite® Plus 488-conjugated KLK3/PSA Recombinant (CL488-84059-5)

    IF Staining of LNCaP using CL488-84059-5

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CoraLite® Plus 488 KLK3/PSA antibody (CL488-84059-5, Clone: 241263F8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated KLK3/PSA Recombinant (CL488-84059-5)

    FC experiment of PC-3 using CL488-84059-5

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody (CL488-84059-5, Clone:241263F8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated KLK3/PSA Recombinant (CL488-84059-5)

    FC experiment of HeLa using CL488-84059-5

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody (CL488-84059-5, Clone:241263F8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated KLK3/PSA Recombinant monoclonal antibody

KLK3/PSA Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-84059-5
Clone No.241263F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inLNCaP cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84059-5 targets KLK3/PSA in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Fusion Protein

相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 3
Calculated molecular weight29 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberNM_001648.2
Gene Symbol KLK3
Gene ID (NCBI) 354
RRIDAB_3673335
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07288-1
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KLK3/PSA antibody CL488-84059-5Download protocol
Standard Protocols
Click here to view our Standard Protocols
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