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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KLHL20 Polyclonal antibody (18111-1-AP)

    WB analysis of HEK-293 using 18111-1-AP (same clone as 18111-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18111-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using KLHL20 Polyclonal antibody (18111-1-AP)

    WB analysis of mouse liver using 18111-1-AP (same clone as 18111-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18111-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using KLHL20 Polyclonal antibody (18111-1-AP)

    WB analysis of HEK-293 using 18111-1-AP (same clone as 18111-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18111-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using KLHL20 Polyclonal antibody (18111-1-AP)

    WB analysis of HEK-293 using 18111-1-AP (same clone as 18111-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18111-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KLHL20 Polyclonal antibody (18111-1-AP)

    IHC staining of human kidney using 18111-1-AP (same clone as 18111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18111-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KLHL20 Polyclonal antibody (18111-1-AP)

    IHC staining of human kidney using 18111-1-AP (same clone as 18111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18111-1-PBS in a different storage buffer formulation.

KLHL20 Polyclonal antibody, PBS Only

KLHL20 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18111-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18111-1-PBS targets KLHL20 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12567

Product name: Recombinant human KLHL20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 261-609 aa of BC063418

Sequence: LLSPKFLVGTVGSDPLIKSDEECRDLVDEAKNYLLLPQERPLMQGPRTRPRKPIRCGEVLFAVGGWCSGDAISSVERYDPQTNEWRMVASMSKRRCGVGVSVLDDLLYAVGGHDGSSYLNSVERYDPKTNQWSSDVAPTSTCRTSVGVAVLGGFLYAVGGQDGVSCLNIVERYDPKENKWTRVASMSTRRLGVAVAVLGGFLYAVGGSDGTSPLNTVERYNPQENRWHTIAPMGTRRKHLGCAVYQDMIYAVGGRDDTTELSSAERYNPRTNQWSPVVAMTSRRSGVGLAVVNGQLMAVGGFDGTTYLKTIEVFDPDANTWRLYGGMNYRRLGGGVGVIKMTHCESHIW

相同性解析による交差性が予測される生物種
Full Name kelch-like 20 (Drosophila)
Calculated molecular weight 609 aa, 68 kDa
Observed molecular weight 65-67 kDa
GenBank accession numberBC063418
Gene Symbol KLHL20
Gene ID (NCBI) 27252
RRIDAB_2131189
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2M5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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