• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL20 Polyclonal antibody

KLHL20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18111-1-AP

Synonyms

kelch like 20 (Drosophila), Kelch like protein 20, Kelch like protein X, KHLHX, KLEIP, KLHL20, KLHLX, RP3 383J4.3

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 18111-1-AP

    WB analysis of HEK-293 using 18111-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 18111-1-AP

    WB analysis of mouse liver using 18111-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18111-1-AP

    WB analysis of HEK-293 using 18111-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18111-1-AP

    WB analysis of HEK-293 using 18111-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18111-1-AP

    IHC staining of human kidney using 18111-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 18111-1-AP

    IHC staining of human kidney using 18111-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18111-1-AP is KLHL20 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL20 fusion protein Ag12567 相同性解析による交差性が予測される生物種
Full Name kelch-like 20 (Drosophila)
Calculated molecular weight 609 aa, 68 kDa
Observed molecular weight 65-67 kDa
GenBank accession numberBC063418
Gene symbol KLHL20
Gene ID (NCBI) 27252
RRIDAB_2131189
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL20 antibody 18111-1-APDownload protocol
IHC protocol for KLHL20 antibody 18111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols