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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLHL15 Polyclonal antibody (25066-1-AP)

    WB analysis of HeLa using 25066-1-AP (same clone as 25066-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using KLHL15 Polyclonal antibody (25066-1-AP)

    WB analysis of HEK-293 using 25066-1-AP (same clone as 25066-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human testis using 25066-1-AP (same clone as 25066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human prostate cancer using 25066-1-AP (same clone as 25066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25066-1-AP (KLHL15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human prostate cancer using 25066-1-AP (same clone as 25066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25066-1-AP (KLHL15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human testis using 25066-1-AP (same clone as 25066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human brain using 25066-1-AP (same clone as 25066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human brain using 25066-1-AP (same clone as 25066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25066-1-PBS in a different storage buffer formulation.

KLHL15 Polyclonal antibody, PBS Only

KLHL15 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25066-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25066-1-PBS targets KLHL15 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16878

Product name: Recombinant human KLHL15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC109058

Sequence: MAGDVEGFCSSIHDTSVSAGFRALYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMFTADMRERDQDKIHLKGLTATGFSHVLQFMYYGTIELSMNTVHEILQAAMYVQLIEVVKFCCSFLLAKICLENCAEIMRLLDDFGVNIEGVREKLDTFLLDNFVPLMSRPDFLSYLSFEKLMSYLDNDHLSRFPEIELYEAVQSWLRHDRRRWRHTDTIIQNIRFCLMTPTSVFEKVKTSEFYRYSRQLRYEVDQALNYFQNVHQQPLLDMKSSRIRSAKPQTTVFRGMIGHSMVNSKILLLKKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVF

相同性解析による交差性が予測される生物種
Full Name kelch-like 15 (Drosophila)
Calculated molecular weight 604 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC109058
Gene Symbol KLHL15
Gene ID (NCBI) 80311
RRIDAB_2879880
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M94
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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