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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL15 Polyclonal antibody

KLHL15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25066-1-AP

Synonyms

kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 25066-1-AP

    WB analysis of HeLa using 25066-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25066-1-AP

    WB analysis of HEK-293 using 25066-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25066-1-AP

    IHC staining of human brain using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 25066-1-AP

    IHC staining of human testis using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 25066-1-AP

    IHC staining of human brain using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 25066-1-AP

    IHC staining of human testis using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25066-1-AP is KLHL15 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL15 fusion protein Ag16878 相同性解析による交差性が予測される生物種
Full Name kelch-like 15 (Drosophila)
Calculated molecular weight 604 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC109058
Gene symbol KLHL15
Gene ID (NCBI) 80311
RRIDAB_2879880
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL15 antibody 25066-1-APDownload protocol
IHC protocol for KLHL15 antibody 25066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Micropeptide PACMP inhibition elicits synthetic lethal effects by decreasing CtIP and poly(ADP-ribosyl)ation.

Authors - Chuanchao Zhang
View Article