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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse brain using 14849-1-AP (same clone as 14849-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse ovary using 14849-1-AP (same clone as 14849-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of rat brain using 14849-1-AP (same clone as 14849-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse heart using 14849-1-AP (same clone as 14849-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse testis using 14849-1-AP (same clone as 14849-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse lung using 14849-1-AP (same clone as 14849-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse liver using 14849-1-AP (same clone as 14849-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of SH-SY5Y using 14849-1-AP (same clone as 14849-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of SH-SY5Y using 14849-1-AP (same clone as 14849-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IF Staining of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14-Specific antibody (14849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KLHL14-Specific Polyclonal antibody, PBS Only

KLHL14-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14849-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14849-1-PBS targets KLHL14-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name kelch-like 14 (Drosophila)
Calculated molecular weight 70 kDa
Observed molecular weight39-45 kDa
GenBank accession numberNM_020805
Gene Symbol KLHL14
Gene ID (NCBI) 57565
RRIDAB_2131171
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2G3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.

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