Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 14849-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14849-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 14849-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14849-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 14849-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 14849-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14849-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14849-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14849-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14849-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 14849-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14849-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 14849-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14-Specific antibody (14849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

KLHL14-Specific Polyclonal antibody

KLHL14-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14849-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse heart tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse ovary tissue, HepG2 cells, rat brain tissue
Positive IHC detected in	human heart tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below

Product Information

14849-1-AP targets KLHL14-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	kelch-like 14 (Drosophila)
Calculated molecular weight	70 kDa
Observed molecular weight	39-45 kDa
GenBank accession number	NM_020805
Gene Symbol	KLHL14
Gene ID (NCBI)	57565
RRID	AB_2131171
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P2G3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.

Protocols

Product Specific Protocols
IF protocol for KLHL14-Specific antibody 14849-1-AP	Download protocol
IHC protocol for KLHL14-Specific antibody 14849-1-AP	Download protocol
WB protocol for KLHL14-Specific antibody 14849-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Clin Exp Pathol Kelch-like protein 14 promotes proliferation and migration of ovarian cancer cells. Authors - Zheng Chen KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14849-1-AP

WB analysis of HepG2 using 14849-1-AP

WB analysis of SH-SY5Y using 14849-1-AP

WB analysis of mouse brain using 14849-1-AP

WB analysis of mouse ovary using 14849-1-AP

WB analysis of SH-SY5Y using 14849-1-AP

WB analysis of HeLa using 14849-1-AP

WB analysis of rat brain using 14849-1-AP

WB analysis of mouse heart using 14849-1-AP

WB analysis of mouse testis using 14849-1-AP

WB analysis of mouse lung using 14849-1-AP

WB analysis of mouse liver using 14849-1-AP

IHC staining of human cervical cancer using 14849-1-AP

IHC staining of human heart using 14849-1-AP