Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 19028-1-AP (same clone as 19028-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 19028-1-AP (same clone as 19028-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
WB analysis of SH-SY5Y using 19028-1-AP (same clone as 19028-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 19028-1-AP (same clone as 19028-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19028-1-AP (KLC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19028-1-AP (KLC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 19028-1-AP (same clone as 19028-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19028-1-AP (KLC1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 19028-1-AP (same clone as 19028-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KLC1 antibody (19028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KLC1 antibody (19028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 19028-1-AP (same clone as 19028-1-PBS)
Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)
Immunofluorescent analysis of HepG2 cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

KLC1 Polyclonal antibody, PBS Only

KLC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19028-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KLC, KLC 1, KNS2, KNS2A

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19028-1-PBS targets KLC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13570 Product name: Recombinant human KLC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-290 aa of BC008881 Sequence: MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLG 相同性解析による交差性が予測される生物種
Full Name	kinesin light chain 1
Calculated molecular weight	65 kDa
Observed molecular weight	61-70 kDa
GenBank accession number	BC008881
Gene Symbol	KLC1
Gene ID (NCBI)	3831
RRID	AB_10641041
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07866
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Kinesin is tetrameric proteins containing two heavy (alpha) chains and two light (beta) chains. The alpha chains provide the tubulin binding site and the ATPase domains, whereas the beta chains are responsible for the specific attachment of the organelle to be moved by the kinesin tetramer (PubMed:21385839).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19028-1-AP (same clone as 19028-1-PBS)

WB analysis of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

WB analysis of COLO 320 using 19028-1-AP (same clone as 19028-1-PBS)

WB analysis of SH-SY5Y using 19028-1-AP (same clone as 19028-1-PBS)

WB analysis of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)

IHC staining of rat brain using 19028-1-AP (same clone as 19028-1-PBS)

IHC staining of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

IHC staining of human bowen disease using 19028-1-AP (same clone as 19028-1-PBS)

IF Staining of HeLa using 19028-1-AP (same clone as 19028-1-PBS)

IF Staining of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)