Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 19028-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19028-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 19028-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 19028-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 19028-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 19028-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 19028-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human bowen disease using 19028-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19028-1-AP (KLC1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 19028-1-AP
Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 19028-1-AP
Immunofluorescent analysis of HepG2 cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HeLa using 19028-1-AP
Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

KLC1 Polyclonal antibody

KLC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19028-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KLC, KLC 1, KNS2, KNS2A

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, COLO 320 cells, HepG2 cells, mouse brain tissue, SK-N-SH cells
Positive IHC detected in	human brain tissue, human bowen disease **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:100-1:600
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 1 publications below

Product Information

19028-1-AP targets KLC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLC1 fusion protein Ag13570 相同性解析による交差性が予測される生物種
Full Name	kinesin light chain 1
Calculated molecular weight	65 kDa
Observed molecular weight	61-70 kDa
GenBank accession number	BC008881
Gene Symbol	KLC1
Gene ID (NCBI)	3831
RRID	AB_10641041
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07866
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Kinesin is tetrameric proteins containing two heavy (alpha) chains and two light (beta) chains. The alpha chains provide the tubulin binding site and the ATPase domains, whereas the beta chains are responsible for the specific attachment of the organelle to be moved by the kinesin tetramer (PubMed:21385839).

Protocols

Product Specific Protocols
WB protocol for KLC1 antibody 19028-1-AP	Download protocol
IHC protocol for KLC1 antibody 19028-1-AP	Download protocol
IF protocol for KLC1 antibody 19028-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	FASEB J Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport. Authors - Amélie Robert KO Validated View Article
human	WB,IF	Dev Neurobiol Forebrain commissure formation in zebrafish embryo requires the binding of KLC1 to CRMP2 Authors - Simo Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19028-1-AP

WB analysis of mouse brain using 19028-1-AP

WB analysis of COLO 320 using 19028-1-AP

WB analysis of SH-SY5Y using 19028-1-AP

WB analysis of HepG2 using 19028-1-AP

IHC staining of human brain using 19028-1-AP