• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using KIF5C Polyclonal antibody (25897-1-AP)

    WB analysis using 25897-1-AP (same clone as 25897-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25897-1-AP (KIF5C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIF5C Polyclonal antibody (25897-1-AP)

    IP experiment of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

    IP result of anti-KIF5C (IP:25897-1-AP, 4ug; Detection:25897-1-AP 1:800) with mouse brain tissue lysate 2080 ug. This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF5C Polyclonal antibody (25897-1-AP)

    IHC staining of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF5C Polyclonal antibody (25897-1-AP)

    IHC staining of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KIF5C Polyclonal antibody (25897-1-AP)

    IF Staining of HeLa using 25897-1-AP (same clone as 25897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIF5C antibody (25897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KIF5C Polyclonal antibody (25897-1-AP)

    IF Staining of SH-SY5Y using 25897-1-AP (same clone as 25897-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KIF5C antibody (25897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

KIF5C Polyclonal antibody, PBS Only

KIF5C Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25897-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

NKHC, KINN, Kinesin-1, Kinesin heavy chain neuron-specific 2, KIAA0531

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25897-1-PBS targets KIF5C in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF5C fusion protein Ag23163 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5C
Calculated molecular weight 725 aa, 83 kDa
Observed molecular weight 70 kDa, 110 kDa
GenBank accession numberBC110287
Gene Symbol KIF5C
Gene ID (NCBI) 3800
RRIDAB_2880288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60282
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KIF5C (Kinesin heavy chain isoform 5C variant) which belongs to the kinesin-like protein family is neuron specific. The kinesin-1 subfamilies (kif5a, kif5b, kif5c) are responsible for the movement of mitochondria in neurons. Kinesins have N-terminal motor domains and C-terminal cargo-binding tail domains separated by hinge regions. The hinge and C-terminal tail regions of Kif5a, Kif5b, and Kif5c bound a large detergent-resistant RNase-sensitive granule. The granule localized to dendrites and underwent bidirectional movement. Distally directed movement of the granule was enhanced by Kif5 overexpression and reduced by Kif5 functional blockage. kinesins may transport RNA in dendrites via this large granule. Two major isoforms of kif5c, with molecular predicted masses of 80 and 110 kDa, are generated by alternative splicing.