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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KIF5B Polyclonal antibody

KIF5B Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21632

Synonyms

KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-21632

    IF Staining of HepG2 using CL488-21632

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 KIF5B antibody (CL488-21632) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 21632-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-21632 targets KIF5B in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF5B fusion protein Ag16250 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5B
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC126279
Gene symbol KIF5B
Gene ID (NCBI) 3799
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KIF5B antibody CL488-21632Download protocol
Standard Protocols
Click here to view our Standard Protocols