Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-27218 targets KIF2A in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25692 Product name: Recombinant human KIF2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 528-679 aa of BC031828 Sequence: VDPTAAGDVRPIMHHPPNQIDDLETQWGVGSSPQRDDLKLLCEQNEEEVSPQLFTFHEAVSQMVEMEEQVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVDSYATQLEAILEQKIDILTELRDKVKSFRAALQEEEQASKQINPKRPRAL 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin heavy chain member 2A |
| Calculated molecular weight | 679 aa, 77 kDa |
| Observed molecular weight | 80 kDa~100 kDa |
| GenBank accession number | BC031828 |
| Gene Symbol | KIF2A |
| Gene ID (NCBI) | 3796 |
| RRID | AB_3672809 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00139 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Kinesin Family Member 2A (KIF2A), also known as Kinesin-2, is a member of the kinesin-13 superfamily protein. KIF2A is a microtubule-depolymerizing kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. KIF2A is involved in microtubule depolymerization at minus ends of the spindle to generate pole-migration forces for chromosome movement. KIF2A is a crucial factor in the invasion and metastasis of various tumors, such as gastric cancer(PMID: 32801897), hepatocellular carcinoma(PMID: 36277155).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 KIF2A antibody CL488-27218 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
