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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF13B Polyclonal antibody (30141-1-AP)

    WB analysis using 30141-1-AP (same clone as 30141-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30141-1-AP (KIF13B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using KIF13B Polyclonal antibody (30141-1-AP)

    WB analysis of HepG2 using 30141-1-AP (same clone as 30141-1-PBS)

    WB result of KIF13B antibody (30141-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF13B transfected HepG2 cells. This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using KIF13B Polyclonal antibody (30141-1-AP)

    IP experiment of HEK-293 using 30141-1-AP (same clone as 30141-1-PBS)

    IP result of anti-KIF13B (IP:30141-1-AP, 4ug; Detection:30141-1-AP 1:800) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KIF13B Polyclonal antibody (30141-1-AP)

    IHC staining of human stomach cancer using 30141-1-AP (same clone as 30141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30141-1-AP (KIF13B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KIF13B Polyclonal antibody (30141-1-AP)

    IHC staining of human stomach cancer using 30141-1-AP (same clone as 30141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30141-1-AP (KIF13B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KIF13B Polyclonal antibody, PBS Only

KIF13B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 30141-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

GAKIN, KIAA0639, kinesin family member 13B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30141-1-PBS targets KIF13B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30876

Product name: Recombinant human KIF13B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1648-1826 aa of NM_015254

Sequence: VRRVRASELRSFSRMLAGDPGCSPGAEGNAPAPGAGGQALASDSEEADEVPEWLREGEFVTVGAHKTGVVRYVGPADFQEGTWVGVELDLPSGKNDGSIGGKQYFRCNPGYGLLVRPSRVRRATGPVRRRSTGLRLGAPEARRSATLSGSATNLASLTAALAKADRSHKNPENRKSWAS

相同性解析による交差性が予測される生物種
Full Name kinesin family member 13B
Calculated molecular weight 203 kDa
Observed molecular weight 250 kDa
GenBank accession numberNM_015254
Gene Symbol KIF13B
Gene ID (NCBI) 23303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQT8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KIF13B, Also known as GAKIN, is a 250-kD phosphoprotein. KIF13B contains a consensus ATP/GTP-binding motif within its N-terminal motor domain; a stalk domain containing several short alpha-helical segments; and a CAP-gly motif in its C terminus characteristic of microtubule-based cytoskeleton components(PMID: 10859302).

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