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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIF13B Polyclonal antibody

KIF13B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30141-1-AP

Synonyms

GAKIN, KIAA0639, KIF13B, kinesin family member 13B, Kinesin like protein GAKIN, Kinesin like protein KIF13B

Validation Data Gallery

Applications:
  • WB analysis using 30141-1-AP

    WB analysis using 30141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30141-1-AP (KIF13B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 30141-1-AP

    IP experiment of HEK-293 using 30141-1-AP

    IP result of anti-KIF13B (IP:30141-1-AP, 4ug; Detection:30141-1-AP 1:800) with HEK-293 cells lysate 1400 ug.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

30141-1-AP targets KIF13B in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF13B fusion protein Ag30876 相同性解析による交差性が予測される生物種
Full Name kinesin family member 13B
Calculated molecular weight 203 kDa
Observed molecular weight 250 kDa
GenBank accession numberNM_015254
Gene symbol KIF13B
Gene ID (NCBI) 23303
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF13B, Also known as GAKIN, is a 250-kD phosphoprotein. KIF13B contains a consensus ATP/GTP-binding motif within its N-terminal motor domain; a stalk domain containing several short alpha-helical segments; and a CAP-gly motif in its C terminus characteristic of microtubule-based cytoskeleton components(PMID: 10859302).

Protocols

Product Specific Protocols
WB protocol for KIF13B antibody 30141-1-APDownload protocol
IP protocol for KIF13B antibody 30141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols