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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated KIAA0284 Monoclonal (CL488-66749)

    FC experiment of HeLa using CL488-66749

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human KIAA0284 (CL488-66749, Clone:1D4B8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated KIAA0284 Monoclonal antibody

KIAA0284 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66749
Clone No.1D4B8

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra)

Synonyms

Cep170B, FAM68C, KIAA0284, Protein KIAA0284

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66749 targets KIAA0284 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen KIAA0284 fusion protein Ag26852 相同性解析による交差性が予測される生物種
Full Name KIAA0284
Calculated molecular weight 1589 aa, 172 kDa
Observed molecular weight 172 kDa
GenBank accession numberBC112928
Gene Symbol KIAA0284
Gene ID (NCBI) 283638
RRIDAB_3084270
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4F5
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 KIAA0284 antibody CL488-66749Download protocol
Standard Protocols
Click here to view our Standard Protocols