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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNH1 Polyclonal antibody (26426-1-AP)

    WB analysis using 26426-1-AP (same clone as 26426-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26426-1-AP (KCNH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26426-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IP experiment of rat brain using 26426-1-AP (same clone as 26426-1-PBS)

    IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with rat brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 26426-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IP experiment of mouse brain using 26426-1-AP (same clone as 26426-1-PBS)

    IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 26426-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IHC staining of mouse brain using 26426-1-AP (same clone as 26426-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26426-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IHC staining of rat brain using 26426-1-AP (same clone as 26426-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26426-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IHC staining of rat brain using 26426-1-AP (same clone as 26426-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26426-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KCNH1 Polyclonal antibody, PBS Only

KCNH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26426-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EAG, EAG channel 1, EAG1, Ether-a-go-go potassium channel 1, h eag

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26426-1-PBS targets KCNH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16647

Product name: Recombinant human KCNH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 686-850 aa of BC113709

Sequence: VKREEEERMKRKNEAPLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGNVLTEHASANHSLVKASVVTVRESPATPVSFQAASTSGVPDHAKLQAPGSECLGPKGGGGDCAKRKSWARFKDACGKSEDWNKVSKAESMETLPERTKASGEATLKKT

相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, subfamily H (eag-related), member 1
Calculated molecular weight 989 aa, 111 kDa
Observed molecular weight111 kDa
GenBank accession numberBC113709
Gene Symbol KCNH1
Gene ID (NCBI) 3756
RRIDAB_3742187
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95259
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Potassium voltage-gated channel subfamily H member 1 (KCNH1, also known as Kv10.1 and EAG) a member of the EAG (ether-à-go-go) family, which is involved in intracellular signaling, cell proliferation, and tumorigenesis (PMID: 35639255). KCNH1 is mainly expressed in the adult central nervous system. It also plays a role in controlling K+ fux that regulates resting membrane potential in excitable and non-excitable cells and is activated at the onset of myoblast differentiation (PMID: 29333676; 27029528; 9738473).

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