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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNH1 Polyclonal antibody (26426-1-AP)

    WB analysis using 26426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26426-1-AP (KCNH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of rat brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IP experiment of rat brain using 26426-1-AP

    IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with rat brain tissue lysate 1720 ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IP experiment of mouse brain using 26426-1-AP

    IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with mouse brain tissue lysate 2120 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IHC staining of mouse brain using 26426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IHC staining of rat brain using 26426-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using KCNH1 Polyclonal antibody (26426-1-AP)

    IHC staining of rat brain using 26426-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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KCNH1 Polyclonal antibody

KCNH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26426-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EAG, EAG channel 1, EAG1, Ether-a-go-go potassium channel 1, h eag

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26426-1-AP targets KCNH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNH1 fusion protein Ag16647 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, subfamily H (eag-related), member 1
Calculated molecular weight 989 aa, 111 kDa
Observed molecular weight111 kDa
GenBank accession numberBC113709
Gene Symbol KCNH1
Gene ID (NCBI) 3756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Potassium voltage-gated channel subfamily H member 1 (KCNH1, also known as Kv10.1 and EAG) a member of the EAG (ether-à-go-go) family, which is involved in intracellular signaling, cell proliferation, and tumorigenesis (PMID: 35639255). KCNH1 is mainly expressed in the adult central nervous system. It also plays a role in controlling K+ fux that regulates resting membrane potential in excitable and non-excitable cells and is activated at the onset of myoblast differentiation (PMID: 29333676; 27029528; 9738473).

Protocols

Product Specific Protocols
WB protocol for KCNH1 antibody 26426-1-APDownload protocol
IHC protocol for KCNH1 antibody 26426-1-APDownload protocol
IP protocol for KCNH1 antibody 26426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols