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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated KAT2A/GCN5 Monoclonal antibody

KAT2A/GCN5 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1F6G7

Cat no : CL750-66575

Synonyms

GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, Lysine acetyltransferase 2A, PCAF b, STAF97

Validation Data Gallery

Applications:
  • WB analysis using CL750-66575

    WB analysis using CL750-66575

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66575 (KAT2A/GCN5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 66575-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

CL750-66575 targets KAT2A/GCN5 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KAT2A/GCN5 fusion protein Ag6940 相同性解析による交差性が予測される生物種
Full Name K(lysine) acetyltransferase 2A
Calculated molecular weight 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC032743
Gene symbol KAT2A
Gene ID (NCBI) 2648
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 KAT2A/GCN5 antibody CL750-66575Download protocol
Standard Protocols
Click here to view our Standard Protocols