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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JAKMIP1 Recombinant antibody (86257-3-RR)

    WB analysis using 86257-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86257-3-RR (JAKMIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using JAKMIP1 Recombinant monoclonal antibody (86257-3-RR)

    IF Staining of Jurkat using 86257-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using JAKMIP1 Recombinant monoclonal antibody (86257-3-RR)

    IF Staining of HEK-293 using 86257-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86257-3-RR

    Affinity and Kinetic Characterization of 86257-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86257-3-RR against Human JAKMIP1 were performed. The affinity constant is below 1 pM.

JAKMIP1 Recombinant monoclonal antibody

JAKMIP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86257-3-RR
Clone No.251026C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GABA-B receptor-binding protein, GABABRBP, JAMIP1, janus kinase and microtubule interacting protein 1, Janus kinase and microtubule-interacting protein 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86257-3-RR targets JAKMIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4816

Product name: Recombinant human JAKMIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 277-626 aa of BC047075

Sequence: VQDQHMDERDVRRFQLKIAELNSVIRKLEDRNTLLADERNELLKRSRETEVQLKPLVEKNKRMNKKNEDLLQSIQRMEEKIKNLTRENVEMKEKLSAQASLKRHTSLNDLSLTRDEQEIEFLRLQVLEQQHVIDDLSLERERLLRSKRHRGKSLKPPKKHVVETFFGFDEESVDSETLSETSYNTDRTDRTPATPEEDLDDATAREEADLRFCQLTREYQALQRAYALLQEQVGGTLDAEREARTREQLQADLLRCQAKIEDLEKLLVEKGQDSKWVEEKQLLIRTNQDLLEKIYRLEMEENQLKNEMQDAKDQNELLEFRVLELEVRDSICCKLSNGADILFEPKLKFM

相同性解析による交差性が予測される生物種
Full Name janus kinase and microtubule interacting protein 1
Calculated molecular weight 73 kDa
Observed molecular weight 73-75 kDa
GenBank accession numberBC047075
Gene Symbol JAKMIP1
Gene ID (NCBI) 152789
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96N16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for JAKMIP1 antibody 86257-3-RRDownload protocol
WB protocol for JAKMIP1 antibody 86257-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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