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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JAKMIP1 Recombinant monoclonal antibody (86257-3-RR)

    WB analysis using 86257-3-RR (same clone as 86257-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86257-3-RR (JAKMIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86257-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using JAKMIP1 Recombinant monoclonal antibody (86257-3-RR)

    IF Staining of Jurkat using 86257-3-RR (same clone as 86257-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86257-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using JAKMIP1 Recombinant monoclonal antibody (86257-3-RR)

    IF Staining of HEK-293 using 86257-3-RR (same clone as 86257-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86257-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86257-3-RR

    Affinity and Kinetic Characterization of 86257-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86257-3-RR against Human JAKMIP1 were performed. The affinity constant is below 1 pM.

JAKMIP1 Recombinant monoclonal antibody, PBS Only

JAKMIP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86257-3-PBS
Clone No.251026C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GABA-B receptor-binding protein, GABABRBP, JAMIP1, janus kinase and microtubule interacting protein 1, Janus kinase and microtubule-interacting protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86257-3-PBS targets JAKMIP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4816

Product name: Recombinant human JAKMIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 277-626 aa of BC047075

Sequence: VQDQHMDERDVRRFQLKIAELNSVIRKLEDRNTLLADERNELLKRSRETEVQLKPLVEKNKRMNKKNEDLLQSIQRMEEKIKNLTRENVEMKEKLSAQASLKRHTSLNDLSLTRDEQEIEFLRLQVLEQQHVIDDLSLERERLLRSKRHRGKSLKPPKKHVVETFFGFDEESVDSETLSETSYNTDRTDRTPATPEEDLDDATAREEADLRFCQLTREYQALQRAYALLQEQVGGTLDAEREARTREQLQADLLRCQAKIEDLEKLLVEKGQDSKWVEEKQLLIRTNQDLLEKIYRLEMEENQLKNEMQDAKDQNELLEFRVLELEVRDSICCKLSNGADILFEPKLKFM

相同性解析による交差性が予測される生物種
Full Name janus kinase and microtubule interacting protein 1
Calculated molecular weight 73 kDa
Observed molecular weight 73-75 kDa
GenBank accession numberBC047075
Gene Symbol JAKMIP1
Gene ID (NCBI) 152789
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96N16
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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