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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated JAB1 Polyclonal antibody

JAB1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27511

Synonyms

COPS5, CSN5, JAB1, MOV 34, SGN5, Signalosome subunit 5

Validation Data Gallery

Applications:
  • FC experiment of HEK-293 using CL488-27511

    FC experiment of HEK-293 using CL488-27511

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human JAB1 (CL488-27511) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy COPS5/JAB1 Ready-To-Use IHC Kit. COPS5/JAB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27511-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-27511 targets JAB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAB1 fusion protein Ag25950 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
Calculated molecular weight 37 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC001187
Gene symbol COPS5
Gene ID (NCBI) 10987
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 JAB1 antibody CL488-27511Download protocol
Standard Protocols
Click here to view our Standard Protocols