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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Islet 1 Polyclonal antibody

Islet 1 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15661

Synonyms

Isl 1, ISL LIM homeobox 1, ISL1, Islet 1, ISLET1

Validation Data Gallery

Applications:
  • IF Staining of SH-SY5Y using CL488-15661

    IF Staining of SH-SY5Y using CL488-15661

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 Islet 1 antibody (CL488-15661) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using CL488-15661

    FC experiment of SH-SY5Y using CL488-15661

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Islet 1 (CL488-15661) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 15661-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-15661 targets Islet 1 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Islet 1 fusion protein Ag8088 相同性解析による交差性が予測される生物種
Full Name ISL LIM homeobox 1
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC031213
Gene symbol ISL1
Gene ID (NCBI) 3670
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.