Validation Data Gallery
Tested Applications
| Positive WB detected in | MOLT-4 cells, Raji cells, U-937 cells, NK-92 cells, THP-1 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67040-1-Ig targets Integrin Alpha 4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28024 Product name: Recombinant human Integrin alpha-4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 653-1032 aa of BC156712 Sequence: SMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD 相同性解析による交差性が予測される生物種 |
| Full Name | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Calculated molecular weight | 1032 aa, 115 kDa |
| Observed molecular weight | 130-150 kDa |
| GenBank accession number | BC156712 |
| Gene Symbol | Integrin alpha 4 |
| Gene ID (NCBI) | 3676 |
| RRID | AB_2882354 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13612 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Alpha 4 antibody 67040-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
