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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin Alpha 4 Monoclonal antibody (67040-1-Ig)

    WB analysis using 67040-1-Ig (same clone as 67040-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Integrin Alpha 4 Monoclonal antibody (67040-1-Ig)

    WB analysis using 67040-1-Ig (same clone as 67040-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NK-92 cells using Integrin Alpha 4 Monoclonal antibody (67040-1-Ig)

    WB analysis of NK-92 using 67040-1-Ig (same clone as 67040-1-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using Integrin Alpha 4 Monoclonal antibody (67040-1-Ig)

    WB analysis of U-937 using 67040-1-Ig (same clone as 67040-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

Integrin Alpha 4 Monoclonal antibody, PBS Only

Integrin Alpha 4 Monoclonal Antibody for WB, ELISA
Cat No. 67040-1-PBS
Clone No.1B6A3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

ITGA4, 1B6A3, CD49 antigen-like family member D, CD49D, IA4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67040-1-PBS targets Integrin Alpha 4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28024

Product name: Recombinant human Integrin alpha-4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 653-1032 aa of BC156712

Sequence: SMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD

相同性解析による交差性が予測される生物種
Full Name integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
Calculated molecular weight 1032 aa, 115 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC156712
Gene Symbol Integrin alpha 4
Gene ID (NCBI) 3676
RRIDAB_2882354
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP13612
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

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