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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Importin Beta Recombinant monoclonal antibody (86630-3-RR)

    WB analysis using 86630-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86630-3-RR (KPNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Importin Beta Recombinant monoclonal antibody (86630-3-RR)

    WB analysis of HeLa using 86630-3-RR

    WB result of Importin Beta antibody (86630-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Beta transfected HeLa cells.

  • Affinity and Kinetic Characterization of 86630-3-RR

    Affinity and Kinetic Characterization of 86630-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86630-3-RR against Human Importin Beta were performed. The affinity constant is 1.02 nM.

  • Featured Product
  • KD/KO Validated

Importin Beta Recombinant monoclonal antibody

Importin Beta Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86630-3-RR
Clone No.251633D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KPNB1, IMB1, Impnb, Importin 90, Importin subunit beta 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA2780 cells, HeLa cells, HT-1080 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86630-3-RR targets Importin Beta in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0122

Product name: Recombinant human KPNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 409-612 aa of BC003572

Sequence: AMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLL

相同性解析による交差性が予測される生物種
Full Name karyopherin (importin) beta 1
Calculated molecular weight 97 kDa
Observed molecular weight 90-97 kDa
GenBank accession numberBC003572
Gene Symbol KPNB1
Gene ID (NCBI) 3837
RRIDAB_3745020
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14974
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

Protocols

Product Specific Protocols
WB protocol for Importin Beta antibody 86630-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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