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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Importin Beta Recombinant monoclonal antibody (86630-3-RR)

    WB analysis using 86630-3-RR (same clone as 86630-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86630-3-RR (KPNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86630-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Importin Beta Recombinant monoclonal antibody (86630-3-RR)

    WB analysis of HeLa using 86630-3-RR (same clone as 86630-3-PBS)

    WB result of Importin Beta antibody (86630-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Beta transfected HeLa cells. This data was developed using the same antibody clone with 86630-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86630-3-RR

    Affinity and Kinetic Characterization of 86630-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86630-3-RR against Human Importin Beta were performed. The affinity constant is 1.02 nM.

  • Featured Product
  • KD/KO Validated

Importin Beta Recombinant monoclonal antibody, PBS Only

Importin Beta Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86630-3-PBS
Clone No.251633D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

KPNB1, IMB1, Impnb, Importin 90, Importin subunit beta 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86630-3-PBS targets Importin Beta in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0122

Product name: Recombinant human KPNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 409-612 aa of BC003572

Sequence: AMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLL

相同性解析による交差性が予測される生物種
Full Name karyopherin (importin) beta 1
Calculated molecular weight 97 kDa
Observed molecular weight 90-97 kDa
GenBank accession numberBC003572
Gene Symbol KPNB1
Gene ID (NCBI) 3837
RRIDAB_3745020
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14974
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

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