Validation Data Gallery
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Tested Applications
| Positive FC detected in | human PBMCs, rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98741-1-MR targets IgG1, λ Isotype Control in FC applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Mouse / IgG1 |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
unknow 相同性解析による交差性が予測される生物種 |
| Full Name | IgG1, λ Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3746308 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
An IgG1, λ isotype control is a critical negative control reagent used in flow cytometry to distinguish specific antibody binding from non-specific background signals. It is an antibody that shares the same host species, immunoglobulin class (IgG1), light chain type (λ), and fluorophore conjugate as the primary antibody used in an experiment, but it lacks specificity for the target antigen.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG1, λ Isotype Control antibody 98741-1-MR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |