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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using ITIH3 Polyclonal antibody (21247-1-AP)

    WB analysis of human plasma using 21247-1-AP (same clone as 21247-1-PBS)

    human serum were subjected to SDS PAGE followed by western blot with 21247-1-AP (ITIH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21247-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using ITIH3 Polyclonal antibody (21247-1-AP)

    IP experiment of human plasma using 21247-1-AP (same clone as 21247-1-PBS)

    IP result of anti-ITIH3 (IP:21247-1-AP, 4ug; Detection:21247-1-AP 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 21247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ITIH3 Polyclonal antibody (21247-1-AP)

    IHC staining of human stomach cancer using 21247-1-AP (same clone as 21247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 21247-1-AP (ITIH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ITIH3 Polyclonal antibody (21247-1-AP)

    IHC staining of human stomach cancer using 21247-1-AP (same clone as 21247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 21247-1-AP (ITIH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21247-1-PBS in a different storage buffer formulation.

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ITIH3 Polyclonal antibody, PBS Only

ITIH3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 21247-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Serum-derived hyaluronan-associated protein, ITI-HC3, ITI heavy chain H3, ITI HC3, Inter-alpha-trypsin inhibitor heavy chain H3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21247-1-PBS targets ITIH3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITIH3 fusion protein Ag15750 相同性解析による交差性が予測される生物種
Full Name inter-alpha (globulin) inhibitor H3
Calculated molecular weight 890 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC107604
Gene Symbol ITIH3
Gene ID (NCBI) 3699
RRIDAB_2878830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06033
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ITIH3, or inter-alpha-trypsin inhibitor heavy chain 3, is a gene in humans that encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex plays a role in stabilizing the extracellular matrix through its ability to bind hyaluronic acid. The gene is located on chromosome 3 and is part of the inter-alpha-trypsin inhibitor family gene cluster. ITIH3 has restricted expression primarily toward the liver, with an RPKM value of 228.8, indicating a relatively high level of expression in this organ.