Validation Data Gallery
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Tested Applications
| Positive WB detected in | human serum tissue |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
21247-1-AP targets ITIH3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ITIH3 fusion protein Ag15750 相同性解析による交差性が予測される生物種 |
| Full Name | inter-alpha (globulin) inhibitor H3 |
| Calculated molecular weight | 890 aa, 100 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC107604 |
| Gene Symbol | ITIH3 |
| Gene ID (NCBI) | 3699 |
| RRID | AB_2878830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06033 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ITIH3, or inter-alpha-trypsin inhibitor heavy chain 3, is a gene in humans that encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex plays a role in stabilizing the extracellular matrix through its ability to bind hyaluronic acid. The gene is located on chromosome 3 and is part of the inter-alpha-trypsin inhibitor family gene cluster. ITIH3 has restricted expression primarily toward the liver, with an RPKM value of 228.8, indicating a relatively high level of expression in this organ.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| IHC protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| IP protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. | ||
J Pers Med Comprehensive Analysis and Experimental Validation of a Novel Estrogen/Progesterone-Related Prognostic Signature for Endometrial Cancer. | ||
Dis Markers Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer. |