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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of HL-60 using 60258-1-Ig

    Untreated and PNGase F-treated lysates of HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of human spleen tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of human spleen using 60258-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of U-937 using 60258-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of THP-1 using 60258-1-Ig

    Untreated and PNGase F-treated lysates of THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of THP-1 using 60258-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of HL-60 using 60258-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human ovary cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human ovary cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human breast cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human breast cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human tonsillitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human tonsillitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human appendicitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human appendicitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CD11c Monoclonal antibody

CD11c Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60258-1-Ig
Clone No.2F1C10
Publications(33)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Synonyms

2F1C10, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman spleen tissue, THP-1 cells, HL-60 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human liver cancer tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human appendicitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60258-1-Ig targets CD11c in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11350

Product name: Recombinant human CD11c/Integrin alpha X protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-352 aa of BC038237

Sequence: NLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMA

相同性解析による交差性が予測される生物種
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
Observed molecular weight 145 kDa
GenBank accession numberBC038237
Gene Symbol CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
RRIDAB_2881379
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Protocols

Product Specific Protocols
IF protocol for CD11c antibody 60258-1-IgDownload protocol
IHC protocol for CD11c antibody 60258-1-IgDownload protocol
WB protocol for CD11c antibody 60258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell

Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer.

Authors - Rodrigo Nalio Ramos
View Article
humanIF

Sci Immunol

Initiator cell death event induced by SARS-CoV-2 in the human airway epithelium

Authors - Kaixin Liang
View Article
humanIF

J Exp Med

AIM2 regulates anti-tumor immunity and is a viable therapeutic target for melanoma.

Authors - Keitaro Fukuda
View Article
humanIF

J Exp Med

PMID: 32181805

Authors - Xiaoyan Zhan
View Article

MedComm (2020)

Plasmacytoid and CD141+ Myeloid Dendritic Cells Cooperation with CD8+ T Cells in Lymph Nodes is Associated with HIV Control

Authors - Joana Vitallé
View Article
humanIF

Diabetes

Loss of IDO1 Expression From Human Pancreatic β-Cells Precedes Their Destruction During the Development of Type 1 Diabetes.

Authors - Florence Anquetil
View Article
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