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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CD11c Monoclonal antibody (60258-1-Ig)

    WB analysis of HL-60 using 60258-1-Ig (same clone as 60258-1-PBS)

    Untreated and PNGase F-treated lysates of HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using CD11c Monoclonal antibody (60258-1-Ig)

    WB analysis of human spleen using 60258-1-Ig (same clone as 60258-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CD11c Monoclonal antibody (60258-1-Ig)

    WB analysis of THP-1 using 60258-1-Ig (same clone as 60258-1-PBS)

    Untreated and PNGase F-treated lysates of THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using CD11c Monoclonal antibody (60258-1-Ig)

    WB analysis of U-937 using 60258-1-Ig (same clone as 60258-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CD11c Monoclonal antibody (60258-1-Ig)

    WB analysis of THP-1 using 60258-1-Ig (same clone as 60258-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CD11c Monoclonal antibody (60258-1-Ig)

    WB analysis of HL-60 using 60258-1-Ig (same clone as 60258-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human breast cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human breast cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IF Staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IF Staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IF Staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IF Staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

CD11c Monoclonal antibody, PBS Only

CD11c Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60258-1-PBS
Clone No.2F1C10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

2F1C10, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60258-1-PBS targets CD11c in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11350

Product name: Recombinant human CD11c/Integrin alpha X protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-352 aa of BC038237

Sequence: NLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMA

相同性解析による交差性が予測される生物種
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
Observed molecular weight 145 kDa
GenBank accession numberBC038237
Gene Symbol CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
RRIDAB_2881379
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20702
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

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