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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IRF1 Recombinant antibody (85254-3-RR)

    WB analysis of HeLa using 85254-3-RR

    HeLa untreated and IFN gamma treated cells lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IRF1 Recombinant antibody (85254-3-RR)

    WB analysis using 85254-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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IRF1 Recombinant antibody

IRF1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85254-3-RR
Clone No.250167D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

interferon regulatory factor 1, IRF 1, IRF-1, MAR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85254-3-RR targets IRF1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IRF1 fusion protein Ag1879 相同性解析による交差性が予測される生物種
Full Name IRF 1
Calculated molecular weight 325 aa, 37 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC009483
Gene Symbol IRF1
Gene ID (NCBI) 3659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Protocols

Product Specific Protocols
WB protocol for IRF1 antibody 85254-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols