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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IRF1 Recombinant antibody (85254-3-RR)

    WB analysis of HeLa using 85254-3-RR (same clone as 85254-3-PBS)

    HeLa untreated and IFN gamma treated cells lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85254-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF1 Recombinant antibody (85254-3-RR)

    WB analysis using 85254-3-RR (same clone as 85254-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85254-3-PBS in a different storage buffer formulation.

IRF1 Recombinant monoclonal antibody, PBS Only

IRF1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85254-3-PBS
Clone No.250167D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

interferon regulatory factor 1, IRF 1, IRF-1, MAR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85254-3-PBS targets IRF1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1879

Product name: Recombinant human IRF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-325 aa of BC009483

Sequence: MPITRMRMRPWLEMQINSNQIPGLIWINKEEMIFQIPWKHAAKHGWDINKDACLFRSWAIHTGRYKAGEKEPDPKTWKANFRCAMNSLPDIEEVKDQSRNKGSSAVRVYRMLPPLTKNQRKERKSKSSRDAKSKAKRKSCGDSSPDTFSDGLSSSTLPDDHSSYTVPGYMQDLEVEQALTPALSPCAVSSTLPDWHIPVEVVPDSTSDLYNFQVSPMPSTSEATTDEDEEGKLPEDIMKLLEQSEWQPTNVDGKGYLLNEPGVQPTSVYGDFSCKEEPEIDSPGGDIGLSLQRVFTDLKNMDATWLDSLLTPVRLPSIQAIPCAP

相同性解析による交差性が予測される生物種
Full Name IRF 1
Calculated molecular weight 325 aa, 37 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC009483
Gene Symbol IRF1
Gene ID (NCBI) 3659
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10914
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

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