Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 22167-1-AP (same clone as 22167-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
WB analysis using 22167-1-AP (same clone as 22167-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
WB analysis of SGC-7901 using 22167-1-AP (same clone as 22167-1-PBS)
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 22167-1-AP (same clone as 22167-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)
IP result of anti-IQGAP1 (IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 22167-1-AP (same clone as 22167-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 22167-1-PBS
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 22167-1-AP (same clone as 22167-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 22167-1-AP (same clone as 22167-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22167-1-AP (IQGAP1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 22167-1-PBS
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug IQGAP1 Polyclonal antibody (22167-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug IQGAP1 Polyclonal antibody (22167-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

IQGAP1 Polyclonal antibody, PBS Only

IQGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 22167-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

HUMORFA01, KIAA0051, p195, Ras GTPase-activating-like protein IQGAP1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22167-1-PBS targets IQGAP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17821 Product name: Recombinant human IQGAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 478-827 aa of BC151834 Sequence: QLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRD 相同性解析による交差性が予測される生物種
Full Name	IQ motif containing GTPase activating protein 1
Calculated molecular weight	189 kDa
Observed molecular weight	190-195 kDa
GenBank accession number	BC151834
Gene Symbol	IQGAP1
Gene ID (NCBI)	8826
ENSEMBL Gene ID	ENSG00000140575
RRID	AB_10949193
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P46940
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22167-1-AP (same clone as 22167-1-PBS)

WB analysis using 22167-1-AP (same clone as 22167-1-PBS)

WB analysis of SGC-7901 using 22167-1-AP (same clone as 22167-1-PBS)

WB analysis of HeLa using 22167-1-AP (same clone as 22167-1-PBS)

IP experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of mouse lung using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of mouse lung using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of human colon using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of human colon using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of human breast cancer using 22167-1-AP (same clone as 22167-1-PBS)

IHC staining of human breast cancer using 22167-1-AP (same clone as 22167-1-PBS)

IF Staining of A549 using 22167-1-PBS

IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)

IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)

IF Staining of A549 using 22167-1-AP (same clone as 22167-1-PBS)

IF Staining of HeLa using 22167-1-AP (same clone as 22167-1-PBS)

IF Staining of HeLa using 22167-1-PBS

FC experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)