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Species Reactivity

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  • Featured Product
  • KD/KO Validated

IQGAP1 Polyclonal antibody

IQGAP1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22167-1-AP

Synonyms

HUMORFA01, IQGAP1, KIAA0051, p195, SAR1

Validation Data Gallery

Applications:
  • WB analysis using 22167-1-AP

    WB analysis using 22167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 22167-1-AP

    WB analysis of HeLa using 22167-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 22167-1-AP

    WB analysis of NIH/3T3 using 22167-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of SGC-7901 using 22167-1-AP

    WB analysis of SGC-7901 using 22167-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 22167-1-AP

    WB analysis of HEK-293 using 22167-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 22167-1-AP

    WB analysis of NIH/3T3 using 22167-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 22167-1-AP

    IP experiment of HeLa using 22167-1-AP

    IP result of anti-IQGAP1 (IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1280 ug.

  • IHC staining of human breast cancer using 22167-1-AP

    IHC staining of human breast cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IHC staining of human breast cancer using 22167-1-AP

    IHC staining of human breast cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IHC staining of human colon cancer using 22167-1-AP

    IHC staining of human colon cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IHC staining of human colon cancer using 22167-1-AP

    IHC staining of human colon cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IHC staining of human liver cancer using 22167-1-AP

    IHC staining of human liver cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IHC staining of human liver cancer using 22167-1-AP

    IHC staining of human liver cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IF Staining of A549 using 22167-1-PBS

    IF Staining of A549 using 22167-1-PBS

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 22167-1-AP

    IF Staining of A549 using 22167-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 22167-1-AP

    IF Staining of HeLa using 22167-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22167-1-AP (IQGAP1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 22167-1-PBS

    IF Staining of HeLa using 22167-1-PBS

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 22167-1-AP

    FC experiment of HeLa using 22167-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IQGAP1 (22167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, SGC-7901 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A549 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IQGAP1 Ready-To-Use IHC Kit. IQGAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

22167-1-AP targets IQGAP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IQGAP1 fusion protein Ag17821 相同性解析による交差性が予測される生物種
Full Name IQ motif containing GTPase activating protein 1
Calculated molecular weight 189 kDa
Observed molecular weight 190-195 kDa
GenBank accession numberBC151834
Gene symbol IQGAP1
Gene ID (NCBI) 8826
RRIDAB_10949193
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

Protocols

Product Specific Protocols
WB protocol for IQGAP1 antibody 22167-1-APDownload protocol
IHC protocol for IQGAP1 antibody 22167-1-APDownload protocol
IF protocol for IQGAP1 antibody 22167-1-APDownload protocol
IP protocol for IQGAP1 antibody 22167-1-APDownload protocol
FC protocol for IQGAP1 antibody 22167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
  • KD Validated
View Article
humanWB

Theranostics

Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.

Authors - Hui-Fang Hu
View Article
WB

Oncogene

The scaffold protein IQGAP1 links heat-induced stress signals to alternative splicing regulation in gastric cancer cells.

Authors - Andrada-Maria Birladeanu
  • KO Validated
View Article
humanWB

Oncotarget

Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1.

Authors - Hyeongsun Moon
View Article
humanWB,IF

Front Cell Infect Microbiol

IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway.

Authors - Mingna Zhao
  • KD Validated
View Article
humanIHC

BMC Cancer

Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma

Authors - Qingqing Dai
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (06-16-2019)

The antibody recognizes IQGAP (top band) but also another band at a lower molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
IQGAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22167-1-AP)
FH

Chun-Chih (Verified Customer) (01-31-2019)

Works well in IP at recommended conc. Clear and strong band, 1mg protein lysate per condition.

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: Human breast cancer cells