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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated INPP5E Polyclonal antibody

INPP5E Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17797

Synonyms

INPP5E, PPI5PIV

Validation Data Gallery

Applications:
  • IF Staining of hTERT-RPE1 using CL488-17797

    IF Staining of hTERT-RPE1 using CL488-17797

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 INPP5E antibody (CL488-17797) at dilution of 1:200.

Tested Applications

Positive IF detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 17797-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-17797 targets INPP5E in IF applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5E fusion protein Ag11959 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Calculated molecular weight 644 aa, 70 kDa
Observed molecular weight 64-66 kDa
GenBank accession numberBC028032
Gene symbol INPP5E
Gene ID (NCBI) 56623
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 INPP5E antibody CL488-17797Download protocol
Standard Protocols
Click here to view our Standard Protocols