Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, hTERT-RPE1 cells, human lung tissue |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
Product Information
66235-1-Ig targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21430 Product name: Recombinant human IL-33 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-270 aa of BC047085 Sequence: MKPKMKYSTNKISTAKWKNTASKALCFKLGKSQQKAKEVCPMYFMKLRSGLMIKKEACYFRRETTKRPSLKTGRKHKRHLVLAACQQQSTVECFAFGISGVQKYTRALHDSSITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 33 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC047085 |
| Gene Symbol | IL-33 |
| Gene ID (NCBI) | 90865 |
| RRID | AB_2881624 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | O95760 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-33 antibody 66235-1-Ig | Download protocol |
| WB protocol for IL-33 antibody 66235-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Oncol Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression. | ||
Cell Cycle Roles of Clara cell 10-kD protein and type 2 innate lymphoid cells in allergic rhinitis. | ||
Sci Rep Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression. | ||
Cells Helicobacter pylori Induces IL-33 Production and Recruits ST-2 to Lipid Rafts to Exacerbate Inflammation. | ||
J Heart Lung Transplant IL-33 mediates Pseudomonas induced airway fibrogenesis and is associated with CLAD | ||
EBioMedicine Polymeric immunoglobulin receptor promotes Th2 immune response in the liver by increasing cholangiocytes derived IL-33: a diagnostic and therapeutic biomarker of biliary atresia |
