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Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using IL-33 Monoclonal antibody (66235-1-Ig)

    WB analysis of human lung using 66235-1-Ig (same clone as 66235-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using IL-33 Monoclonal antibody (66235-1-Ig)

    WB analysis of THP-1 using 66235-1-Ig (same clone as 66235-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of hTERT-RPE1 cells using IL-33 Monoclonal antibody (66235-1-Ig)

    WB analysis of hTERT-RPE1 using 66235-1-Ig (same clone as 66235-1-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IL-33 Monoclonal antibody (66235-1-Ig)

    IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IL-33 Monoclonal antibody (66235-1-Ig)

    IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

IL-33 Monoclonal antibody, PBS Only

IL-33 Monoclonal Antibody for WB, ELISA
Cat No. 66235-1-PBS
Clone No.2A9C7

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, ELISA

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66235-1-PBS targets IL-33 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21430

Product name: Recombinant human IL-33 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-270 aa of BC047085

Sequence: MKPKMKYSTNKISTAKWKNTASKALCFKLGKSQQKAKEVCPMYFMKLRSGLMIKKEACYFRRETTKRPSLKTGRKHKRHLVLAACQQQSTVECFAFGISGVQKYTRALHDSSITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET

相同性解析による交差性が予測される生物種
Full Name interleukin 33
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC047085
Gene Symbol IL-33
Gene ID (NCBI) 90865
RRIDAB_2881624
Conjugate Unconjugated
Form
FormLiquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDO95760
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

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