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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL18RAP Polyclonal antibody (30587-1-AP)

    WB analysis using 30587-1-AP (same clone as 30587-1-PBS)

    Mouse spleen tissue lysate was subjected to SDS PAGE followed by western blot with 30587-1-AP (IL18RAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IP experiment of mouse spleen using 30587-1-AP (same clone as 30587-1-PBS)

    IP result of anti-IL18RAP (IP:30587-1-AP, 4 ug; Detection:30587-1-AP 1:600) with mouse spleen tissue lysate 1920 ug. This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of mouse spleen using 30587-1-AP (same clone as 30587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of mouse spleen using 30587-1-AP (same clone as 30587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of rat spleen using 30587-1-AP (same clone as 30587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of rat spleen using 30587-1-AP (same clone as 30587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of human small intestine using 30587-1-AP (same clone as 30587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of human small intestine using 30587-1-AP (same clone as 30587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30587-1-PBS in a different storage buffer formulation.

IL18RAP Polyclonal antibody, PBS Only

IL18RAP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 30587-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Accessory protein like, Accessory protein-like, ACPL, CD218 antigen-like family member B, CD218b

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30587-1-PBS targets IL18RAP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30327

Product name: Recombinant human IL-18RAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-336 aa of BC069630

Sequence: FNISGCSTKKLLWTYSTRSEEEFVLFCDLPEPQKSHFCHRNRLSPKQVPEHLPFMGSNDLSDVQWYQQPSNGDPLEDIRKSYPHIIQDKCTLHFLTPGVNNSGSYICRPKMIKSPYDVACCVKMILEVKPQTNASCEYSASHKQDLLLGSTGSISCPSLSCQSDAQSPAVTWYKNGKLLSVERSNRIVVDEVYDYHQGTYVCDYTQSDTVSSWTVRAVVQVRTIVGDTKLKPDILDPVEDTLEVELGKPLTISCKARFGFERVFNPVIKWYIKDSDLEWEVSVPEAKSIKSTLKDEIIERNIILEKVTQRDLRRKFV

相同性解析による交差性が予測される生物種
Full Name interleukin 18 receptor accessory protein
Calculated molecular weight 599 aa, 68 kDa
Observed molecular weight70 kDa
GenBank accession numberBC069630
Gene Symbol IL18RAP
Gene ID (NCBI) 8807
RRIDAB_3742353
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95256
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Interleukin-18 receptor accessory protein (IL18RAP, also known as IL1R7 and CD218b) is a crucial component of the IL-18 receptor complex. It enhances the binding activity of IL-18 to its receptor and plays a significant role in IL-18 signaling (PMID: 24842757). IL18RAP is related to the human tumor microenvironment and promotes various immune cell infiltration (PMID: 37698530).

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