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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL18RAP Polyclonal antibody (30587-1-AP)

    WB analysis using 30587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30587-1-AP (IL18RAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of rat spleen using 30587-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of mouse spleen using 30587-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of mouse spleen using 30587-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using IL18RAP Polyclonal antibody (30587-1-AP)

    IHC staining of rat spleen using 30587-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IL18RAP Polyclonal antibody

IL18RAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30587-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Accessory protein like, Accessory protein-like, ACPL, CD218 antigen-like family member B, CD218b

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, K-562 cells, RAW 264.7 cells, rat spleen tissue
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30587-1-AP targets IL18RAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL18RAP fusion protein Ag30327 相同性解析による交差性が予測される生物種
Full Name interleukin 18 receptor accessory protein
Calculated molecular weight 599 aa, 68 kDa
Observed molecular weight60 kDa
GenBank accession numberBC069630
Gene Symbol IL18RAP
Gene ID (NCBI) 8807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95256
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-18 receptor accessory protein (IL18RAP, also known as IL1R7 and CD218b) is a crucial component of the IL-18 receptor complex. It enhances the binding activity of IL-18 to its receptor and plays a significant role in IL-18 signaling (PMID: 24842757). IL18RAP is related to the human tumor microenvironment and promotes various immune cell infiltration (PMID: 37698530).

Protocols

Product Specific Protocols
WB protocol for IL18RAP antibody 30587-1-APDownload protocol
IHC protocol for IL18RAP antibody 30587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols