Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84165-5-RR targets IL-7 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Product name: HumanKine® recombinant human IL-7 protein Source: -derived, Tag: Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 7 |
| Gene Symbol | IL-7 |
| Gene ID (NCBI) | 3574 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13232 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-7 (IL-7) is a non-redundant, pleiotropic cytokine fundamental to the development, homeostasis, and function of the immune system. It is primarily produced by stromal cells in the bone marrow and thymus, as well as by epithelial cells in peripheral lymphoid organs. IL-7 signals through a heterodimeric receptor consisting of the private IL-7 receptor alpha chain (IL-7Rα/CD127) and the common gamma chain (γc), activating key pathways such as JAK/STAT, PI3K/Akt, and MAPK to promote lymphocyte survival, proliferation, and metabolic fitness. Its critical role is underscored by the severe lymphopenia and immunodeficiency observed in both IL-7- and IL-7R-deficient humans and mice.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-7 antibody 84165-5-RR | Download protocol |
| WB protocol for IL-7 antibody 84165-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |