CoraLite® Plus 647-conjugated IL-6 Monoclonal antibody
IL-6 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
4G3E6
Cat no : CL647-69001
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | LPS and Brefeldin A treated HUVEC cells, HUVEC cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL647-69001 targets IL-6 in FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-6 fusion protein HZ-1019 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 6 (interferon, beta 2) |
Gene symbol | IL6 |
Gene ID (NCBI) | 3569 |
RRID | AB_2920319 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
This antibody is used to Block/Neutralize IL6 protein.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 IL-6 antibody CL647-69001 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |