NeutraKine® IL-6 Monoclonal antibody

NeutraKine® IL-6 Monoclonal Antibody for FC, IHC, Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, Neutralization, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4G3E6

Cat no : 69001-1-Ig

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6, NeutraKine® IL-6



Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.
Neutralization

Product Information

69001-1-Ig targets NeutraKine® IL-6 in WB, Neutralization, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IL-6 protein HZ-1019
Full Name interleukin 6 (interferon, beta 2)
Gene symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Lyophilized Powder
Purification Method Protein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

This antibody can be used to neutralize the bioactivity of IL-6.

Protocols

Product Specific Protocols
IHC protocol for NeutraKine® IL-6 antibody 69001-1-IgDownload protocol
FC protocol for NeutraKine® IL-6 antibody 69001-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanNeutralization

J Exp Clin Cancer Res

N-acetyl cysteine induces quiescent-like pancreatic stellate cells from an active state and attenuates cancer-stroma interactions.

Authors - Haimin Feng
human,mouseWB

Biomed Pharmacother

Interleukin-6 neutralizing antibody attenuates the hypersecretion of airway mucus via inducing the nuclear translocation of Nrf2 in chronic obstructive pulmonary disease.

Authors - Yuan-Yuan Wei
  • KD Validated
humanWB

Int J Biol Sci

The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC.

Authors - Wentao Liu