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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50008-1

    Sandwich ELISA standard curve of MP50008-1

    Sandwich ELISA standard curve of MP50008-1, IL-28A Monoclonal Matched Antibody Pair - PBS only. 60270-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1235. 68669-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP50008-1

    Sample test of MP50008-1

    HUVEC cells were cultured unstimulated or stimulated with 100 ng/mL IFN-a and 10 μg/mL Poly I:C for 3 hours. The mean IL-28A concentration was undetectable in unstimulated supernatant, 70.0 pg/mL in stimulated supernatant.

  • Sample test of MP50008-1

    Sample test of MP50008-1

    A549 cells were cultured unstimulated or stimulated with 10 μg/mL Poly I:C and 5 μg/mL Lip for 1 day. The mean IL-28A concentration was undetectable in unstimulated supernatant, 3,774.2 pg/mL in stimulated supernatant.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human small intestine using 60270-1-Ig (same clone as 60270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human small intestine using 60270-1-Ig (same clone as 60270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IF Staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IF Staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

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IL-28A Monoclonal antibody, PBS Only (Capture)

IL-28A Monoclonal Antibody for IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 60270-1-PBS
Clone No.3B4A1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL28A, Cytokine Zcyto20, IFN lambda 2, IFNL2, IFN-lambda-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60270-1-PBS targets IL-28A as part of a matched antibody pair:

MP50008-1: 60270-1-PBS capture and 68669-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.77 x 109M
KOff=4.10 x 10-5M
KOn=2.31 x 104M
Immunogen IL-28A fusion protein Ag18525 相同性解析による交差性が予測される生物種
Full Name interleukin 28A (interferon, lambda 2)
Calculated molecular weight 200 aa, 22 kDa
GenBank accession numberBC113583
Gene Symbol IL-28A
Gene ID (NCBI) 282616
RRIDAB_2881390
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IZJ0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.