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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50008-1

    Sandwich ELISA standard curve of MP50008-1

    Sandwich ELISA standard curve of MP50008-1, IL-28A Monoclonal Matched Antibody Pair - PBS only. 60270-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1235. 68669-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP50008-1

    Sample test of MP50008-1

    A549 cells were cultured unstimulated or stimulated with 10 μg/mL Poly I:C and 5 μg/mL Lip for 1 day. The mean IL-28A concentration was undetectable in unstimulated supernatant, 3,774.2 pg/mL in stimulated supernatant.

  • Sample test of MP50008-1

    Sample test of MP50008-1

    HUVEC cells were cultured unstimulated or stimulated with 100 ng/mL IFN-a and 10 μg/mL Poly I:C for 3 hours. The mean IL-28A concentration was undetectable in unstimulated supernatant, 70.0 pg/mL in stimulated supernatant.

IL-28A Monoclonal antibody, PBS Only (Detector)

IL-28A Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68669-1-PBS
Clone No.1A5C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL28A, Cytokine Zcyto20, IFN lambda 2, IFNL2, IFN-lambda-2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68669-1-PBS targets IL-28A as part of a matched antibody pair:

MP50008-1: 60270-1-PBS capture and 68669-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=5.94 x 10-9M
KOff=3.48 x 10-4M
KOn=5.86 x 104M
Immunogen

CatNo:

Product name: HumanKine® recombinant human IL-28A protein

Source: -derived,

Tag:

Domain: of

Sequence:

相同性解析による交差性が予測される生物種
Full Name interleukin 28A (interferon, lambda 2)
Gene Symbol IL-28A
Gene ID (NCBI) 282616
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8IZJ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.