Validation Data Gallery
Tested Applications
| Positive WB detected in | Transfected with human IL-24 CHO cells |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87821-1-RR targets IL-24 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4971 Product name: Recombinant Human IL-24 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 52-206 aa of BC009681 Sequence: QEFHFGPCQVKGVVPQKLWEAFWAVKDTMQAQDNITSARLLQQEVLQNVSDAESCYLVHTLLEFYLKTVFKNYHNRTVEVRTLKSFSTLANNFVLIVSQLQPSQENEMFSIRDSAHRRFLLFRRAFKQLDVEAALTKALGEVDILLTWMQKFYKL 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 24 |
| Calculated molecular weight | 207 aa, 24 kDa |
| GenBank accession number | BC009681 |
| Gene Symbol | IL-24 |
| Gene ID (NCBI) | 11009 |
| RRID | AB_3745770 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13007 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-24 (IL-24) , also known as Melanoma Differentiation-Associated gene 7 (mda-7) , is a protein that belongs to the IL-10 family of cytokines. It was initially discovered in 1995 by researchers Paul B. Fisher and colleagues as a gene that is induced during the terminal differentiation of melanoma cells. Unlike many other cytokines, IL-24 is secreted primarily by activated monocytes, macrophages, and T helper 2 (Th2) cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-24 antibody 87821-1-RR | Download protocol |
| WB protocol for IL-24 antibody 87821-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |