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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-18 Recombinant antibody

IL-18 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H14

Cat no : 82907-1-RR

Synonyms

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18

Validation Data Gallery

Applications:
  • WB analysis using 82907-1-RR

    WB analysis using 82907-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82907-1-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82907-1-RR

    IF Staining of HeLa using 82907-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (82907-1-RR, Clone: 1H14 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inA549 cells, U2OS cells, HeLa cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IL18 Ready-To-Use IHC Kit. IL18 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:125-1:500
Sample-dependent, check data in validation data gallery

Product Information

82907-1-RR targets IL-18 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL-18 fusion protein Ag1063 相同性解析による交差性が予測される生物種
Full Name IL-18
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC007461
Gene symbol IL18
Gene ID (NCBI) 3606
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Protocols

Product Specific Protocols
WB protocol for IL-18 antibody 82907-1-RRDownload protocol
IF protocol for IL-18 antibody 82907-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols