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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-18 Recombinant monoclonal antibody (82907-1-RR)

    WB analysis using 82907-1-RR (same clone as 82907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82907-1-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IL-18 Recombinant monoclonal antibody (82907-1-RR)

    WB analysis of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

    WB result of IL-18 antibody (82907-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18 transfected HeLa cells. This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-18 Recombinant monoclonal antibody (82907-1-RR)

    IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-18 Recombinant monoclonal antibody (82907-1-RR)

    IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IL-18 Recombinant monoclonal antibody (82907-1-RR)

    IF Staining of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (82907-1-RR, Clone: 1H14 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using IL-18 Recombinant monoclonal antibody (82907-1-RR)

    FC experiment of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IL-18 Recombinant antibody (82907-1-RR, Clone:1H14) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82907-1-RR

    Affinity and Kinetic Characterization of 82907-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82907-1-RR against Human IL-18 were performed. The affinity constant is 0.769 nM.

  • Featured Product
  • KD/KO Validated

IL-18 Recombinant monoclonal antibody, PBS Only

IL-18 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82907-1-PBS
Clone No.1H14

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

IL18, Pre-IL18, 1H14, Iboctadekin, IFN-gamma-inducing factor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82907-1-PBS targets IL-18 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1063

Product name: Recombinant human Pre IL-18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-193 aa of BC007461

Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

相同性解析による交差性が予測される生物種
Full Name IL-18
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC007461
Gene Symbol IL-18
Gene ID (NCBI) 3606
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14116
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.

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