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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using IKZF5 Polyclonal antibody (24780-1-AP)

    WB analysis of Jurkat using 24780-1-AP (same clone as 24780-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IKZF5 Polyclonal antibody (24780-1-AP)

    WB analysis of HeLa using 24780-1-AP (same clone as 24780-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using IKZF5 Polyclonal antibody (24780-1-AP)

    WB analysis of HL-60 using 24780-1-AP (same clone as 24780-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24780-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using IKZF5 Polyclonal antibody (24780-1-AP)

    IP experiment of Jurkat using 24780-1-AP (same clone as 24780-1-PBS)

    IP result of anti-IKZF5 (IP:24780-1-AP, 4ug; Detection:24780-1-AP 1:300) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 24780-1-PBS in a different storage buffer formulation.

IKZF5 Polyclonal antibody, PBS Only

IKZF5 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 24780-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24780-1-PBS targets IKZF5 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20405

Product name: Recombinant human IKZF5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 140-376 aa of BC022564

Sequence: CELCSFRCSDRSNLSHHRRRKHKMVPIKGTRSSLSSKKMWGVLQKKTSNLGYSRRALINLSPPSVVVQKPDYLNDFTHEIPNIQTDSYESMAKTTPTGGLPRDPQELMVDNPLNQLSTLAGQLSSLPPENQNPASPDVVPCPDEKPFMIQQPSTQAVVSAVSASIPQSSSPTSPEPRPSHSQRNYSPVAGPSSEPSAHTSTPSIGNSQPSTPAPALPVQDPQLLHHCQHCDMYFADN

相同性解析による交差性が予測される生物種
Full Name IKAROS family zinc finger 5 (Pegasus)
Calculated molecular weight 420 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC022564
Gene Symbol IKZF5
Gene ID (NCBI) 64376
RRIDAB_2879717
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H5V7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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