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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IKZF5 Polyclonal antibody

IKZF5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24780-1-AP

Synonyms

IKZF5, PEGASUS, Zinc finger protein Pegasus, ZNFN1A5

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 24780-1-AP

    WB analysis of Jurkat using 24780-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 24780-1-AP

    WB analysis of HL-60 using 24780-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24780-1-AP

    WB analysis of HeLa using 24780-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 24780-1-AP

    IP experiment of Jurkat using 24780-1-AP

    IP result of anti-IKZF5 (IP:24780-1-AP, 4ug; Detection:24780-1-AP 1:300) with Jurkat cells lysate 2800ug.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IP detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy IKZF5 Ready-To-Use IHC Kit. IKZF5 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24780-1-AP is IKZF5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IKZF5 fusion protein Ag20405 相同性解析による交差性が予測される生物種
Full Name IKAROS family zinc finger 5 (Pegasus)
Calculated molecular weight 420 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC022564
Gene symbol IKZF5
Gene ID (NCBI) 64376
RRIDAB_2879717
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IKZF5 antibody 24780-1-APDownload protocol
IP protocol for IKZF5 antibody 24780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols